Glioblastoma multiforme (GBM) is the most malignant primary brain tumor. Resident microglia and peripheral infiltrating macrophages account for up to half of the non-neoplastic cells in a GBM. These tumor-associated macrophages have been implicated in proliferation, angiogenesis, and immunosuppression in GBM and can influence the efficacy of chemo-, radio-, and immunotherapies.
However, certain cancer specific interactions have been associated with either microglia or macrophages, necessitating an approach that can delineate the two populations. Multiplex immunofluorescence offers a technical advantage that allows for the profound phenotyping of cells in the tumor microenvironment as well as their spatial organization.
In this poster we show you how we designed a multiplex immunofluorescence protocol to differentiate microglia and infiltrating M1/M2 macrophages in GBM in situ.