Complex flow cytometry is critical for today’s personalized medicine. With 25 years of extensive expertise in providing flow cytometry testing for clinical trials ranging from Phase I to Phase III, Cerba Research has a strong track record of developing and validating custom-made flow cytometry panels. While many of these are drug-specific and proprietary to the sponsor, we also supply off-the-shelf options.

At Cerba Research, we develop, validate, qualify, and implement these assays on a global scale. With capacity in Europe, Asia-Pacific, the U.S., Australia, and South Africa, we are a leading central lab known for our expertise in flow cytometry methodology.

All our instruments are standardized and state-of-the-art, with up to 12 channels for optimal testing and 24-color machines coming soon. Global standardization procedures and acceptance criteria defined on our 8- and 10-color equipment ensure consistency and reproducibility. Our fully integrated clinical trial management system (CTMS) secures data transfer, from test request to results reporting.

State-of-the-art instruments for flow cytometry

  • BD FACSCanto™ II
  • Navios – Beckman Coulter

Scientist-to-scientist interaction yields optimal panel design and project-adapted reportables

Our dedicated flow cytometry team works with you to develop fit-for-purpose assays — validated panels in a multitude of diseases. These practiced experts interpret your complex clinical and safety data, offering incomparable value with proven excellence in improving the quality of assays.

Tailored solutions solve complex challenges and optimize your workflow

From panel design to review of data, you can obtain customized methodologies to reliably measure:

  • Immune cell phenotypes
  • Immune cell activation
  • Receptor occupancy and density
  • Hematologic malignancies
  • Output in relative percentages, absolute count, and fluorescence

In addition to customized panels, we provide numerous off-the-shelf options, including a multiple myeloma panel. For further details and since new tests are added frequently, please reach out for our complete list of biomarkers.

Flow Cytometry Biomarkers
‘X’CAR-TAny CAR-T target ‘X’Drug target
Bcl2Apoptosis regulatorApoptosis regulator
BCMAB cells, plasma cellsCheckpoint, immune cell regulation
CCR7Memory and naive T cellsImmunophenotyping: T cell activation
CD4T helper, expressed by monocytes and macrophagesImmunophenotyping: T cell activation
CD5T cells, B cellsImmunophenotyping: T cell suppression
CD8T cells, NK cellsImmunophenotyping: cytotoxic T cell = activated T cell
CD11bMonocytes, MDSCs, neutrophils and macrophages, T cells, B cells, dendritic cellsImmunophenotyping: T cell activation
CD14Monocytes, macrophages, monocytic MDSCs, granulocytesImmune cell activation
CD15Granulocytes, macrophages and monocytesImmune cell activation
CD16T cells, dendritic cells, NK cells, macrophages and monocytes, granulocytesImmune cell activation
CD19B cells, dendritic cells, stem cellsImmune cell regulation
CD25T cells, B cells, NK cells, macrophages and monocytesImmune cell activation
CD27T cells, B cells, NK cellsImmune cell activation
CD28T cellsT cell proliferation
CD31T cells, B cells, NK cells, macrophages and monocytes, granulocytes, plateletsImmune cell activation
CD33Monocytes, MDSCs, neutrophils, macrophagesCell adhesion and signal transduction
CD38T cells, B cells, dendritic cells, NK cells, stem cells, macrophages and monocytesCell adhesion and signal transduction
CD39T cells, B cells, NK cells, macrophages and monocytesImmune cell regulation
CD45Activated and memory T cells, some B cells subsets, activated monocytes and macrophages, granulocytes, NK cells, dendritic cellsImmune cell regulation
CD47T cells, B cells, NK cells, macrophages and monocytes, granulocytes, plateletsCell adhesion and signal transduction
CD52T cells, B cells, NK cells, macrophages and monocytesImmunophenotyping: cytotoxicity
CD56T cells, NK cellsCell adhesion
CD69T cells, B cells, NK cells, macrophages and monocytes, granulocytes, plateletsImmune cell proliferation and signal transduction
CD86T cells, B cells, dendritic cells, macrophages and monocytesT cell activation
CD117Stem cells/precursorImmunophenotyping: cell adhesion immune cells
CD123Dendritic cells, stem cells, granulocytesImmune cell differentiation
CD127T cells, stem cells, macrophages and monocytesImmune cell activation
CD137Activated T cells, NK cellsCheckpoint: immune cell regulation
CD138B cells, stem cellsImmune cell activation, cell proliferation
CD314T cells, B cells, NK cells, macrophages and monocytes, granulocytesImmune cell regulation
CLL1Car-T targetDrug target
CXCR3T cellsT cell activation
CXCR4Leukocytes, tumor cellsCell migration
HLAAntigen presenting cellsImmune cell activation
KappaSurface markerDiagnosis and identification of hematopoietic malignancies
Ki67Tumor cellsTumor cell proliferation and growth
LAG3Activated T cellsCheckpoint, immune cell regulation
LambdaSurface markerDiagnosis and identification of hematopoietic malignancies
OX40Activated T cells, NK cells, neutrophilsImmune cell activation and differentiation
PD-1Activated T cells, B cellsCheckpoint: immune cell regulation
PD-L1Tumor cells and immune cells including T cells, B cells and antigen presenting cellsCheckpoint: immune cell regulation
RQR8B cellsSafety switch
TIM3T cellsCheckpoint: immune cell regulation
Flow Cytometry Panels
Basic Immune Phenotyping Panels
  • TBNK (CD4/CD8 T cells, B cells, NK cells)
  • T cell subsets (CD4/CD8 T cells)
T Cell Phenotyping Panels

T cell backbone (CD45/CD3/CD4/CD8)

  • T cell activation (CD25/CD69/CD38)
  • Regulatory T cells (CD25/CD127)
  • T cell exhaustion (PD1/TIM3/LAG3)
  • T cell proliferation and survival (Ki-67/Bcl-2)
  • Naive and memory T cells (CD45RO/CD45RA/CD62L/)
B and NK Cell Panels
  • B cell activation (CD45/viability/CD19/CD20/CD59/CD10/CD5)
  • NK cell subsets (CD45/viability/CD3/CD16/CD56)
  • NK cell activation (CD45/viability/CD3/CD16/CD56/CD158a;b/CD314/CD335)
Other Immune Cell Panels
  • Monocyte and neutrophil (CD45, CD14/CD15/CD11b) 
  • Extensive panel (CD16/CD64/CD300e)
  • Monocytic MDSC (CD45/lineage/HLA-DR/CD11b/CD33/CD14/CD15)
  • pDC and basophil (CD45/HLA-DR/CD123/BDCA2)
Multiple Myeloma Panel

Contact us for more information